Composite

Part:BBa_K1911001:Design

Designed by: Jack Kwon   Group: iGEM16_LambertGA   (2016-10-06)


pLac-ClpXP-CI


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1096
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 578
    Illegal AgeI site found at 1316
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The pLac-ClpXP-CI system was built so that illegal restriction sites were not present in our sequences so that other teams wishing to use the system may be able to use it and easily combine it with other sequences.

Source

The pLac-ClpXP-CI system is not found in any genomic sequence. It was built using parts found on the iGEM registry and from certain parts found in E. coli cells (i.e ClpXP)

References